CHARACTERISTICS OF SOME HYDROLYTIC ENZYMES AND BIOPULPING POTENTIAL OF FUNGAL ISOLATES FROM SELECTED WOOD SAMPLES IN SOUTHWESTERN NIGERIA
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
Abstract
Pulping industry is both labour and energy intensive but in Nigeria, electricity supply is unstable. This has contributed to the near collapse of the Nigerian paper and pulp industry despite the rich forest resources in southwestern Nigeria. Use of alternative energy sources such as electricity generating sets further increases production cost. It therefore, becomes pertinent to seek alternative approach aimed at reducing mechanical pulping duration and improving pulp characteristics. The aim of the study was to characterise major hydrolytic enzymes of native fungi from selected wood samples and evaluate their biopulping potential. Chipped samples of three local woods (Anogeissus leiocarpus, Gmelina arborea and Terminalia superba) from Ekiti, Lagos, Ogun, Ondo, Osun and Oyo States were made to undergo spontaneous degradation. Thereafter, fungi were isolated, identified and screened for cellulase and hemicellulase production. Isolates selected based on maximal enzyme activities were used to produce cellulases (glucanase and glucosidase) and hemicellulases (mannanase and xylanase) with the chips as substrates. Kinetic, molecular and biochemical properties of partially purified hemicellulases were determined and optimised using standard methods. Chipped wood samples were subjected to treatments with fungal isolates singly and in combination for six weeks. Treated wood samples and pulp were evaluated fortnightly for changes in cellulose, hemicellulose, lignin and ash contents, tensile strength, fibre length and strength. Data were analysed using descriptive statistics and ANOVA at p = 0.05. Five hundred and twenty-six fungal isolates were obtained and identified as Aspergillus flavus(13), Aspergillus niger(125), Rhizopus spp(79). Lasiodiplodia theobromae(10), Penicillium pur-purogenum(25), Fusarium chlamydosporium(37), Fusarium oxysporum(30), Fusarium compa ctum(25), Trichoderma harzianum(75), Trichoderma reesei(88) and Emericella nidulans(19). Aspergillus niger, F. compactum, T. harzianum and T. reesei were selected for enzyme production. Highest production of cellulase was 12.72U/gm±0.11 on A. leiocarpus by T. reesei. Best producer of mannanase was A. niger with 15.50U/gm±0.01 on G. arborea while highest xylanase production of 28.93U/gm±0.12 was by F. compactum on A. leiocarpus. Kinetic properties of xylanase was Vmax 1.402U/min/ml and Km 1.804U/ml with molecular weight of about 90kDa and that of mannanase was Vmax 0.754U/min/ml, Km 1.364U/ml and molecular weight was between 53 and 65kDa. Optimum xylanase activity was at 55oC and pH 5.5 while mannanase was at 30oC and pH 5.5. There was significant reduction in hemicellulose (45- 27%) and lignin (20-15%) in treated wood compared to untreated samples. Gmelina arborea singly treated with F. compactum gave best result: cellulose, hemicellulose, lignin and ash were reduced by 14.1, 18.9, 4.1 and 4.7 % respectively; while tensile strength, fibre length and strength improved respectively by 6.4, 4.3 and 6.5 %. These conformed to Technical Association of Paper and Pulp Industries standard. Treatment of T. superba with combination of F. compactum and T. harzianum however, reduced cellulose, hemicellulose, lignin and ash by 20.7, 39.4, 11.9 and 10.6 % respectively, giving a better result than that obtained with single treatment. Fungal enzyme treated wood samples attained pulp characteristics that met required biopulping standard. Thus, native fungi may be employed for biopulping of common woods in Nigerian paper and pulp industries.
Related Project Materials
ROLE OF ICT IN THE REFERENCE SERVICES TO LIBRARY USERS IN NIGERIA
Abstract
This research work is on the role of information and communication technology (ICT) in the pro...
DESIGN AND IMPLEMENTATION OF A CUSTOMER RELATIONSHIP MANAGEMENT SYSTEM
3 BACKGROUND THE OF STUDY
Customer Relationship Management (CRM) system came into existence when...
THE EFFECTS OF NEWS COMMERCIALIZATION ON THE CONTENTS OF PRIVATE
Abstract
The research examines the effects of news commercialization on the contents of private media” with a case...
INFLUENCE OF EARLY CHILDHOOD EDUCATION ON DIETARY DIVERSITY
ABSTRACT: This study examines the influence of early childhood education on dietary diversity among young children. Early exposure to dietary...
PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF Mangifera indica (opioro mango)
INTRODUCTION
Origin: Mango (Mangifera indica) (Opioro) is a...
PERFORMANCE EVALUATION OF MINI SPRINKLER IRRIGATION SYSTEM: COMPARISON OF THE PLASTIC AND IRON MADE SPRINKLER SYSTEM
ABSTRACT
This study is a study to evaluate and compare the hydraulic performance of plastic and iron mini sprinkler system using 8mm and...
CYTOLOGICAL EVALUATION OF SPUTUM SAMPLES IN WELDERS IN OWERRI METROPOLIS
ABSTRACT
Metal fume fever and pneumosiderosis is an occupational Lung disease acquired when welders are exposure to weld...
IMPACT OF ADVERTISING ON CONSUMER PATRONAGE
Abstract
Advertising is an economic promoter with the attribute of persuasiveness. In formativeness has an important con...
THE USES OF COMMITTEES AND GROUP MEETINGS IN ENHANCING DECISION MAKING IN NIGERIA PUBLIC SERVICE, (A CASE STUDY OF UNIVERSITY BASIC EDUCATION BOARD, AWKA, ANAMBRA STATE)
ABSTRACT
THE MAJOR PURPOSE OF THIS STUDY IS TO EXAMINE THE USES OF COMMITTEES AND GROUP MEETINGS IN ENHANCING DECISION M...
SOCIAL MEDIA AND THE MANAGEMENT OF COVID-19 INFORMATION IN PORT HARCOURT
ABSTRACT
This study was carried out on social media and the management of covid-19 information in Port Harcourt, Rivers...
